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SOLVED: Laemmli buffer, also referred to as SDS-reducing buffer, is added to each of the samples. It is a blue solution and the recipe for 2X Laemmli buffer is: 0.125 M Tris
2x Tris Glycine SDS Sample buffer (Laemmli, 50 ml) [SAB01-02] - $30.00 : Bioland Scientific, for Your Research Needs